ISOLATION AND IDENTIFICATION OF BACTERIA PRESENT OVER THE MOBILE PHONES WHICH SERVE AS A VEHICLE TO TRANSMIT THE INFECTIONS IN INDIVIDUALS

Author: Mr. Ganesh Waghule.

Abstract:
In this study a total of 50 mobile samples from students and employees were included in this
study. Out of 50 samples collected from mobile phones 24, 14, 9 and 3 samples belong to
students, staff members, cleaners and health professionals respectively. Mobile phones of
students and employees were randomly sampled by taking written and oral consents from all the
participants included in this study. Sampled mobile phone swab was streaked onto nutrient agar.
The inoculated plates were incubated aerobically in an inverted position at 37 °C for 48 hours. 14
colonies selected and sub-cultured to isolate pure culture, After isolating pure cultures, bacterial
isolates were further identified by Gram staining, Mac‐Conkey agar and Biochemical tests were
performed on pure culture for final identification of the isolates on the basis of their biochemical
reaction calculating the total percentage of each isolate, we found E. coli, E. aerogenes,
Streptococci and Staphylococcus aureus in the percentage of 25.16%, 21.26%, 16.17% and
37.28% respectively, The percentage of bacteria identified in our study was found higher than
other study, this study showed that mobile phones would serve as a vehicle to transmit these
bacteria from one individual to another.


Keywords: Mobile phones, nutrient agar, Mac-Conkey agar, Bacteria, E. coli, E.aerogenes.

Citation: 

http://ajptr.com/archive/volume-10/october-2020-issue-5